Skip to main content
. 2019 Aug;14(8):1430–1437. doi: 10.4103/1673-5374.253529

Figure 3.

Figure 3

NO and TNF-α production with cell viability of RAW 264.7 macrophages activated with (A) LPS + IFN-γ, (B) IFN-γ and (C) CEA-AGE.

RAW 264.7 macrophages were activated with LPS (10 μg/mL) + IFN-γ (10 U/mL (1 U = 0.1 ng/mL), IFN-γ (25 U/mL) or CEA-AGE (400 μg/mL). NO and TNF-α production were determined in the media after 24 hours of incubation with increasing concentrations of catalase. Results represent the mean ± SEM of two experiments in triplicate. Statistical analysis was carried out using a one-way analysis of variance followed by Dunnett’s multiple comparison tests (*P < 0.05, **P < 0.01, and ***P < 0.001, vs. positve control (activated cells in the absence of catalase). NO: Nitric oxide; TNF: tumor necrosis factor; LPS: lipopolysaccharide; IFN-γ: interferon-gamma; CEA-AGE: chicken egg albumin-derived advanced glycation end product.