Figure 5.

Combination of HPV peptide vaccine, α4–1BB and αCTLA-4 induces regression of HPV⁺ tongue tumors. Mice were challenged with mEER tumor cells (40,000) in the tongue and treated with the different vaccine and immunotherapy combinations starting on day 5 as described in Fig. 1. A representative MRI (T2-weighted sagittal image) of mouse tongue (A) and tumor volume measured by MRI (B) at day 19 after tumor implantation are shown (data shown are means ± SEM; n = 5–12 mice/group). Survival curves of mice bearing tongue tumors treated with different immunotherapy combinations are shown (C; n = 10 – 23 mice/group). Statistical significance was calculated using one-way ANOVA. *, P < 0.05. Results represent pooled data from multiple experiments.