Table 2.
Summary of proteomic coverages and numbers of hits observed in the STEPP-LiP MCF-7 vs MCF-10A comparative analysis.
| Double Digestiona | Single Digestionb | |
|---|---|---|
| Total peptide (protein) identified | 3650 (1050) | 16656 (1604) |
| Assayed peptide (protein) | 2977 (688)c | |
| Peptide (protein) hits | 1240 (440)d | |
a
The identified peptides in the double digestion group are all semi-tryptic.
b
The identified peptides in the single digestion group are all tryptic.
c
The number of peptides (proteins) identified in least two biological replicates of both the MCF-7 and MCF-10A cell line analyses and in both the double and single digestion groups.
d
The number of semi-tryptic peptides (proteins) with significantly different normalized TMT reporter ion intensities between the MCF-7 and MCF-10A groups (p < 0.05).