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. 2019 May 10;10:1041. doi: 10.3389/fimmu.2019.01041

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Copyright © 2019 Ulivieri, De Tommaso, Finetti, Ortensi, Pelicci, D'Elios, Ballerini and Baldari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rai negatively controls the contact-dependent and -independent upregulation of CD39 and CD73 elicited by encephalitogenic T cells. (A,B) Flow cytometric analysis of surface CD73 and CD39 in Rai^+/+ (Astro WT) and Rai^−/− (Astro Rai^−/−) astrocytes untreated (-) or stimulated for 120 h with culture supernatants from MOG-specific T cells generated from WT mice (SN T-MOG). Data are presented as mean ± SD of mean fluorescence intensity (MFI) (A) or frequency of CD39^highCD73^high astrocytes (n > 3) (B). (C) Flow cytometric analysis of surface CD73 and CD39 expression in Rai^+/+ (Astro WT) and Rai^−/− (Astro Rai^−/−) astrocytes treated for 120 h with either culture supernatants from MOG-specific T cells generated from WT mice (SN T-MOG) or MOG-specific T cells depleted of their culture supernatants (T-MOG) in the presence (+Ag) or absence of MOG antigen. Representative dot plots are shown. The histograms show the frequency of the CD39^highCD73^high population. Data are presented as mean ± SD of the percentage of CD39^highCD73^high cells (n > 3). Representative dot plots are shown. Two-Way ANOVA, ^****p < 0.0001, ^***p < 0.001, ^**p < 0.01, ^*p < 0.05.