Skip to main content
. 2019 May 10;10:1041. doi: 10.3389/fimmu.2019.01041

Figure 6.

Figure 6

Rai negatively controls the conversion of astrocytes toward a neuroprotective phenotype. (A) Real-Time PCR analysis of A1-specific (H2-D1, Serping and C3; upper panels) or A2-specific (Emp1 and S100a10; lower panels) transcripts in WT (Astro WT) and Rai−/− (Astro Rai−/−) astrocytes either untreated or treated for 24 h with culture supernatants from MOG-specific T cells generated from WT mice. Data from 3 independent experiments, each carried out on the pooled astrocytes from at least 5 Rai+/+ or 5 Rai−/− mice, are presented as mean value ± SD. The levels of the different transcripts were normalized to GAPDH, used as housekeeping gene. (B) Real-Time PCR analysis of the A1-specific transcripts H2-D1, Serping and C3 in the brain of Rai+/+ EAE mice (EAE WT) and Rai−/− EAE mice (EAE Rai−/−) 15 days post immunization. Data are presented as mean value ± SD obtained on three Rai+/+ and three Rai−/− EAE mice. 2-way ANOVA; **p < 0.01, *p < 0.05.