Fig. 5.

Biophysical properties of single-chain antibodies with high and low specificity. (A) Apparent melting temperature (T_m^*) and (B) non-native solubility analysis of scFv antibodies with different HCDR3 net charges and different levels of specificity. In (B), the concentration of soluble antibody was measured after heating at 65°C for 4 h and cooling to 4°C overnight (initial antibody concentration of ~0.4 mg/mL). Average values are reported for (A) two or (B) three independent experiments, and the error bars are standard errors.