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. 2019 Mar 18;1(1):56–66. doi: 10.1089/bioe.2018.0005

FIG. 5.

FIG. 5.

Elevated Pi rescues calcification in depolarized hMSCs undergoing OS differentiation. (A) Total calcium content in hMSCs was quantified after 21 days of OS differentiation under depolarized (HK, 40 mM high K+) or nondepolarized (OS) conditions, with 5 mM Pi supplementation for the first 5 days of culture. Different letters over the bars represent statistically different groups as determined by one-way ANOVA and the Tukey–Kramer post hoc test (p < 0.05). Groups (a), (b), and (c) were statistically different from one another, but the samples within group (a) were not statistically different from one another. (B) Total calcium content in hMSCs was quantified under the same conditions with the addition of fosc. Data points represent mean normalized calcium content (μg Ca/μg DNA) ± standard deviation, n = 3–6. Different letters over the bars represent statistically different groups as determined by one-way ANOVA and the Tukey–Kramer post hoc test (p < 0.05). Groups (a), (b), (c), and (d) were statistically different from one another. (C) Intracellular Pi concentration was measured in depolarized (HK) or nondepolarized (OS) cells with or without Pi supplementation (Pi). Asterisks (*) represent significant differences relative to the corresponding depolarized or nondepolarized groups without supplemented Pi, p < 0.05. fosc, foscarnet; Pi, inorganic phosphate.