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. 2019 Apr 30;10:401. doi: 10.3389/fgene.2019.00401

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Copyright © 2019 Zhao, Liu, Xin, Shi, Bai, Sun, Zhao, Wang, Liu, Zhao, Chen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes of thymus, spleen tissue structure, and number of lymphocytes in Rag2/IL2rg^-/- gene double-knockout mice. Six-week mutated and wild-type BALB/c mice were sacrificed. Various organs were extracted, and the organ/weight ratio calculated (A,B). Spleen and thymus tissues were sectioned and stained with the H&E method (C). Peripheral blood was collected from tail veins and, after dilution, sample were stained with FITC-CD3, PE-NKp46, and APC-220 antibodies and detected by flow cytometry FCM (D). Data are presented in the format of mean ± standard deviation of 3 independent experiments performed in triplicate (^∗p ≤ 0.05, ^∗∗p ≤ 0.01, compared with controls).