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. 2019 Apr 30;10:401. doi: 10.3389/fgene.2019.00401

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Copyright © 2019 Zhao, Liu, Xin, Shi, Bai, Sun, Zhao, Wang, Liu, Zhao, Chen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune response level induced by MTB 85B antigen. Recombinant 85B protein was immunized 3 times, the serum and spleen cells collected, and anti-85B specific antibody titer was detected by ELISA (A,B). Spleen lymphocytes’ stimulation index (SI) was measured by MTS (C). IL-2, IL-10, and IFN-γ concentrations in immunized serum were assayed by commercial ELISA kit (D,E,F). Data are presented in the format of mean ± standard deviation of 3 independent experiments performed in triplicate (^∗p ≤ 0.05, ^∗∗p ≤ 0.01, compared with controls).