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. 2019 Apr 30;10:401. doi: 10.3389/fgene.2019.00401

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Copyright © 2019 Zhao, Liu, Xin, Shi, Bai, Sun, Zhao, Wang, Liu, Zhao, Chen and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Replication of human tumor xenograft model in Rag2/IL2rg^-/- gene double-knockout mice. U87 cells were stereotaxically injected to the precuneus to replicate orthotopic transplantation of glioma. Tumor formation and histopathology structure were detected by anatomy and H&E staining (A). Raji cells were injected intravenously into mutated mice and tumor formation was observed at different times by use of the IVIS Lumina II imaging system (B). Primary gastric, renal, and bladder carcinoma cells were implanted subcutaneously in the flank site and bred for 3 weeks, and tumor formation was observed at different time by IVIS Lumina II imaging system (C).