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. 2019 May 7;10:919. doi: 10.3389/fmicb.2019.00919

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Copyright © 2019 Tietzel, Quayle and Carabeo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of inclusion size of mϕ without and with reversal of growth restriction following IL-10 pre-treatment. The photomicrographs were analyzed with ImageJ for size of individual inclusions, graphed in Microsoft Excel, followed by statistical analysis. Macrophages were treated with human interleukin-10 at 48 h (48 h), at 24 h (24 h) before infection, simultaneously with infection (0 h), or not treated at all with IL-10 (none). Resting macrophages are symbolized by circles, CA mϕ by squares, and AA mϕ by triangles. Means are indicated by horizontal bars. Inclusion size for CA mϕ for 0 h are different than 48 h with statistical significance. p-values equal or less than the significance of 0.1 are symbolized by (#), p-values ≤ 0.05 by one asterisk (^∗), p-values ≤ 0.01 are labeled (^∗∗), and p-values ≤ 0.002 are referenced as (^∗∗∗).