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. 2019 May 7;10:919. doi: 10.3389/fmicb.2019.00919

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Copyright © 2019 Tietzel, Quayle and Carabeo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recovery of inclusion forming unit (IFU) reduced in CA mϕ, but not in AA mϕ. (A) Quantitative analysis of recovery of IFU from lysates of resting (Rest), classically activated (CA) or alternatively activated mϕ (AA) that were originally inoculated with a MOI of one (white bars) or five (black bars), respectively. IFU numbers for CA mϕ are dramatically reduced in comparison to resting mϕ with a value of p ≤ 0.01 (^∗) indicating statistical significance. No statistically significant differences were found for AA mϕ when compared to resting mϕ. Confocal micrograph of HeLa cells with immunofluorescently labeled IFUs recovered from resting (B), classically activated (C) or alternatively activated mϕ (D) that were inoculated at an MOI of one. The white scale bar indicates 10 μm.