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. 2019 May 7;10:919. doi: 10.3389/fmicb.2019.00919

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Copyright © 2019 Tietzel, Quayle and Carabeo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High transferrin receptor (TfR) level and growth of chlamydial inclusions in AA mϕ, but not CA cells. (A) Confocal micrographs of infected macrophages pulsed for 1 h with immunofluorescent transferrin (red). Chlamydia trachomatis was detected with indirect immunofluorescent antibodies (green). Detectable transferrin levels for AA mϕ > resting mϕ > CA mϕ. The white scale bar indicates 10 μm. (B) The Fiji release of ImageJ was used to analyze the levels of fluorescently labeled TfR of resting macrophages (Rest), classically activated (CA) and alternatively activated (AA) mϕ in micrographs obtained by confocal laser microscopy. The mean fluorescence of individual cells derived from the mean “gray” value of the red channel were computed in ImageJ. Scatter plots of individual resting macrophages (circular symbol, n: 58 cells), CA macrophages (square symbol, n: 13 cells), and AA macrophages (triangular symbol, n: 28 cells), together with the mean value for each group (horizontal bar) are depicted. The mean value for Rest mϕ was 74.51843, for CA mϕ was 72.11838, and for AA mϕ was 111.5917. One-way ANOVA analysis determined a statistically significant difference between the three groups with a p-value of 0.00000004429. A Tukey Honest Significant Difference post hoc test identified that the differences of TfR levels between AA mϕ and Rest mϕ are statistically significant, as well as between AA mϕ and CA mϕ with p-values below 0.0001 (symbolized as asterisk ^∗). The TfR levels between CA mϕ and Rest mϕ was not found different in a statistically significant way.