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. 2019 Apr 30;10:784. doi: 10.3389/fimmu.2019.00784

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Copyright © 2019 Fu, Senouthai, Wang and You.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Annexin V-FITC and PI staining to evaluate apoptosis in HK-2 cells following different treatment. The HK-2 cells were divided into nine groups and incubated for 48 h with annexin V-FITC and PI and analyzed using flow cytometry. (A) Q4: In each panel the lower left quadrant shows cells that are negative for both PI and annexin V-FITC. Q3: Upper right quadrant shows annexin positive cells (early apoptotic). Q2: Upper left quadrant shows only PI positive cells, which are necrotic. Q1: Lower right quadrant shows annexin and PI positive cells (late apoptotic cells). (a) control group; (b) Ang II group; (c) XAV939 group; (d) FKN-KD group; (e) Ang II + FKN-KD group; (f) XAV939 + FKN-KD group; (g) Ex-FKN group; (h) Ang II + Ex-FKN group; (i) XAV939 + Ex-FKN group.(B) The rate of early apoptotic cells (Q3) is represented in a histogram. p value represents ^*p < 0.05 compared with the control group;# p < 0.05 compared with Ang II group; ## p < 0.05 compared with XAV939 group; ### p < 0.05 compared with FKN-KD group; ** p < 0.05 compared with Ex-FKN group. Data are expressed as the means ± standard deviation (n = 3). Statistical analyses were performed using multi-way classification ANOVA.