Figure 2.

WNT/CTNNB1 Signaling Regulates the Progenitor Fate of Human MGE

(A) Western blotting confirmed the complete lack of CTNNB1 protein expression in knockout (KO) hESCs.

(B) WT- and KO-MGE progenitors at day 25 were subjected to RNA-seq. Volcano plot distinguished 400 upregulated and 560 downregulated differentially expressed genes (DEGs) in KO-MGE progenitors. DEGs with a fold change ≥1.5 and p < 0.05 were marked in red (upregulated) and green (downregulated).

(C and D) GO analyses of DEGs identified the functional annotations of upregulated genes (C) and downregulated genes (D) in KO-MGE progenitors.

(E) Dox-inducible CTNNB1 S33Y overexpression (OE) hESCs were established through lentiviral infection. Western blotting validated the effectiveness of CTNNB1 S33Y overexpression after Dox (0.1 μg/mL) treatment for 5 days.

(F) RNA-seq was performed in day 25-CTNNB1 S33Y OE-MGE progenitors treated with or without Dox from days 17 to 25. Volcano plot distinguished 996 upregulated and 925 downregulated DEGs in Dox-treated OE-MGE progenitors. DEGs with a fold change ≥1.5 and p < 0.05 were marked in red (upregulated) and green (downregulated).

(G and H) GO analyses identified the functional annotations of downregulated genes (G) and upregulated genes (H) in the Dox-treated group.

See also Figures S1 and S2.