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. 2019 May 17;14(5):e0216949. doi: 10.1371/journal.pone.0216949

Fig 1. The expression of EboGP in HIV VLPs facilitates virus entry in MDMs and MDDCs, but not in CD4+ T cells.

Fig 1

A) Schematic structures of various plasmids. a, CMV-Gag-Pol, HIV gp(T/M), EboGP plasmids. CMV PROM: Cytomegalovirus promoter. b, HIV-1 RT, IN and Env deletion provirus ΔRI/ΔE/Gluc. RT: Reverse transcriptase; IN: Integrase; Env: Envelope; Gluc: Gaussia luciferase. B) The presence of EboGP or HIV gp in HIV VLPs. 293T cells were transfected by HIV-1ΔRI/ΔE/Gluc+, CMV-Gag-Pol and HIV gp or EbovGPwt/ΔM. Supernatant containing VLPs were collected, purified, lysed and analyzed by WB with anti-HIVgp, anti-EboGP antibody or anti-HIVp24 antibody. C-E) CD4+ C8166 T cells, human MDMs and human MDDCs were infected by various HIVgp or EboGP pseudotyped HIV VLPs. After 3 days of infection, the supernatants were collected and subjected to Gluc activity assay. Error bars represent variation between duplicate samples and the data is representative of results obtained in three independent experiments.