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. 2019 May 17;14(5):e0216949. doi: 10.1371/journal.pone.0216949

Fig 2. The EboGPΔM incorporation into HIV VLPs mediates more efficient virus entry in MDMs and MDDCs than EboGP.

Fig 2

A) Schematic diagram of the peptide sequence for EboGPwt and EboGPΔM, which contained a deletion of the MLD region. SP: signal peptide; RBD: receptor binding domain; MLD: mucin-like domain; IFL: internal fusion loop; HR1, HR2: heptadrepeat1/2; TM: transmembrane domain. B) Analysis of the viral compositions of various VLPs produced from 293T cells co-transfected with HIV Env(M) and/or EboGP/EboGPΔM expressing plasmids, and HIV CMVin-Gag/Pol and HxΔRI/ΔE/Gluc. Viral compositions were analyzed by WB with anti-HIVgp, anti-EboGP antibody or anti-HIVp24 antibody C) THP1 cells were infected by various HIVgp and/or EboGPwt/ΔM pseudotyped HIV VLPs. At 1, 3, 5, and 7 days post-infection, the supernatants were collected and subjected to Gluc activity and p24 ELISA assay. D) Human MDMs (left panel) or human MDDCs (right panel) were infected by various HIVgp and/or EboGPwt/ΔM pseudotyped HIV VLPs and Gluc activity was monitored at 5 and 10 days post-infection. Error bars represent variation between duplicate samples, and the data is representative of results obtained in two independent experiments.