Table 1. Primers used for RT-PCR.
| Genes | Primer sequences | Expected product size (bp) |
|---|---|---|
| IL-1α |
F-5'-TGAGTCGGCAAAGAAATCAA-3' R-5'-AGAGAGATGGTCAATGGCAGA-3' |
108 |
| IL-1β |
F-5'-TGGGCTGGACTGTTTCTAATG-3' R-5'-GGTTTCTTGTGACCCTGAGC-3' |
134 |
| IL-6 |
F-5'-GAGGATACCACTCCCAACAGACC-3' R-5'-AAGTGCATCATCGTTGTTCATACA-3’ |
141 |
| IL-12p35 |
F-5'-TTGATGATGACCCTGTGCCT-3' R-5'-GATTCTGAAGTGCTGCGTTGA-3' |
91 |
| IL-4 |
F-5'-AAGGGATACAGGGGCTCACT-3' R-5'-CAGGTCTATTCGGTGGACAAA-3' |
121 |
| IL-10 |
F-5'-TGGACAACATACTGCTAACCGAC-3' R-5'-ATGCTCCTTGATTTCTGGGC-3' |
143 |
| iNOS |
F-5'-GAAAAGTCCAGCCGCACCAC-3' R-5'-GGACAATCCACAACTCGCTCC-3' |
149 |
| COX-2 |
F-5'-CTGCCAATAGAACTTCCAATCC-3' R-5'-CGGTTTGATGTTACTGTTGCTT-3' |
134 |
| GAPDH |
F-5'-AGGAGCGAGACCCCACTAACA-3' R-5'-AGGGGGGCTAAGCAGTTGGT-3' |
247 |