2iL/I/F Enhancers from Various 2iL/I/F Culture Conditions
ChIP-seq of naive cells grown in different culture conditions including Elf1 2iL/I/F (navy), Elf1 4iL/I/F (purple), Elf1 AF (transitioning; cyan), primed H1 mTeSR (orange), H1 4iL/I/F (red), and LIS1 4iL/I/F (green).
(A) H3K4me1 enrichment in different growth conditions at DNMT3L and SOX2 loci.
(B) Average ChIP-seq signal at 2iL/I/F-specific enhancers (top panel) and primed-specific enhancers (bottom panel).
(C) Heatmap of H3K4me1 ChIP-seq signal at 2iL/I/F-specific enhancers, defined as not in TR or primed. Scale is RPKM.
(D) PCA of top 500 10-kb bins of H3K4me1 with largest variance.