Figure 7.

Active Elimination of Transplanted NPCs

(A) Experimental design.

(B) Enhanced episomal cloning and expression vector used for diphtheria toxin (DT) receptor insertion.

(C) mCherry⁺ NPCs in bright field (BF) and with red fluorescence filter (C1–C4). Scale bars, 50 μm.

(D) mCherry⁺GFP⁺ NPCs following transplantation into SCI (D1–D4). Scale bars, 20 μm.

(E) GFP⁺ NPCs following transplantation into SCI (E1–E4). Scale bars, 10 μm.

(F) Expression of cytokines/chemokines in the cerebrospinal fluid (CSF) as log₂(fold change) to expression in healthy animals. Each dot represents one animal. p values for two-group comparison between SCI+NPC and SCI+NPC and DT are presented.

(G) BBB locomotor rating scale over time. Data reporting and statistics as in (F) except that 95% CIs are provided instead of box plots (^∗p < 0.05; ns, not significant).

(H) Iliac crest height over time. Data reporting and statistics as in (G). Technical replicates are reported as diamonds.

See also Figure S6.