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. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Lab Invest. 2018 Oct 23;99(1):93–106. doi: 10.1038/s41374-018-0137-1

Figure 1: Immune cell distribution of T cells in C57BL/6NCr, BALB/cAnNCr and 129/SvHsd male and female mice.

Figure 1:

Cell suspensions from BM and spleen were isolated from 8-week old mice. The cells were stained with CD3 and CD4 antibodies for CD4+ T cells, CD3 and CD8 antibodies for CD8+ T cells, and CD3, CD4, CD25 and FoxP3 antibodies for T-regs. The cells were then subjected to flow cytometry to determine cell-type percentages. (A) CD4+ T cells in BM, (n≥8). (B) CD4+ T cells in the spleen, (n≥8). (C) CD8+ T cells in BM, (n≥5). (D) CD8+ T in spleen, (n≥6). (E) CD4+ T-regs in BM, (n≥8). (F) CD4+ T-regs in spleen, (n≥7). Results, shown as scatter plots, depict average cell percentages (percent of live cells). Error bars denote SEM. Each dot represents the value from a single mouse. (♂) and (♀) represent male and female mice, respectively.*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p≤0.0001.