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. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Lab Invest. 2018 Oct 23;99(1):93–106. doi: 10.1038/s41374-018-0137-1

Figure 3: Immune cell distribution of immature myeloid cells (iMCs), macrophages and myeloid dendritic cells (mDCs) in C57BL/6NCr, BALB/cAnNCr and 129/SvHsd for male and female mice.

Figure 3:

Cell suspensions from BM and spleen were isolated from 8-week old mice. The cells were stained with CD11b, F4/80 and CD68 antibodies for macrophages, CD11b and Gr-1 antibodies for iMCs, and CD11b and CD11c antibodies for mDCs. Ly6B was used as negative marker for iMCs. The cells were then subjected to flow cytometry to determine cell-type percentages. (A) iMCs in BM, (n≥7). (B) iMCs in the spleen, (n≥7). (C) Macrophages in BM, (n≥ 6). (D) Macrophages in spleen, (n≥9) (E) mDCs in BM, (n ≥ 10). (F) mDCs in the spleen, (n ≥ 13). Results are shown as scatter plots depicting average cell percentages (percent of live cells). Error bars denote SEM. Each dot represents the value from a single mouse. (♂) and (♀) represent male and female mice, respectively. **p ≤ 0.01, ***p ≤ 0.001, ****p≤0.0001.