Skip to main content
. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Lab Invest. 2018 Oct 23;99(1):93–106. doi: 10.1038/s41374-018-0137-1

Figure 5: Immune cell distribution of neutrophils in C57BL/6NCr, BALB/cAnNCr and 129/SvHsd for male and female mice.

Figure 5:

Cell suspensions from BM and spleen were isolated from 8-week old mice. The cells were stained with CD11b, Gr-1 and Ly6b antibody. F4/80 antibody was included as a negative marker for neutrophils. The cells were then subjected to flow cytometry to determine cell-type percentages. (A) Neutrophils in BM, (n≥7). (B) Neutrophils in spleen, (n ≥7). Results are shown as scatter plots depicting average cell percentages (percent of live cells). Error bars denote SEM. Each dot represents the value from a single mouse. (♂) and (♀) represent male and female mice respectively.*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p≤0.0001.