Table 2.
Details of antibodies used in the study
| Antibody | Fluorochrome | Vendor | Clone | Catalogue # | Antibody Concentration (in 100 pL FACS buffer) |
|---|---|---|---|---|---|
| CD3e | APC-Cy7 | Fisher Scientific | 145–2C11 | BDB557596 | 1 pL |
| CD4 | eFluor450 | eBioscience | GK1.5 | 48–0041-82 | 1 pL |
| CD8 | FITC | eBioscience | 53–6.7 | 11–0081-82 | 0.6 pL |
| CD11b | Vio Blue | Miltenyi | M1/70.15.11.5 | 130–097-336 | 3.75 pL |
| CD11c | PE | Miltenyi | N418 | 130102545 | 3.75 pL |
| CD19 | PE | Miltenyi | 6D5 | 130–102-598 | 5 pL |
| CD25 | PE | eBioscience | PC61.5 | 12–0251-81 | 0.5 pL |
| CD68 | PE | Miltenyi | FA-11 | 130–102-614 | 3.75 pL |
| CD80 | APC | Miltenyi | 16–10A1 | 130–102-584 | 3.75 pL |
| CD86 | FITC | Miltenyi | PO3.3 | 130–102-506 | 5 pL |
| B220 | APC-Vio770 | Miltenyi | RA3–6B2 | 130–102-267 | 3.75 pL |
| F4/80 | APC | Miltenyi | REA126 | 130–102-379 | 3.75 pL |
| FoxP3 | APC | eBioscience | FJK-16s | 17–5773-82 | 5 pL |
| Gr1 | APC-eFluor780 | eBioscience | RB6–8C5 | 47–5931-80 | 1.2 pL |
| Ly6b | FITC | abcam | 7/4 | ab53453 | 0.65 pL |
| NKp46 | FITC | Miltenyi | 29A1.4.9 | 130–102-300 | 3.75 pL |
| SiglecH | FITC | eBioscience | eBio440c | 11–0333-82 | 0.5 pL |