Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Catalogue no. | Additional information |
|---|---|---|---|---|
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) myc-RanWT-CYFP | this study | Used in BiFC | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) NYFP-RanWT-HA |
this study | Used in BiFC | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) myc-RanCmut-CYFP | this study | Used in BiFC | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) NYFP-RanCmut-HA |
this study | Used in BiFC | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) CFP-RanWT |
this study | Used in FRET | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) myc-RanWT | this study | Used in FRET | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) hRanBP1Δlinker-NLS | this study | Used in FRET | |
| Genetic reagent (H. sapiens) |
pcDNA 3.1(+) hRanBP1ΔNES-NLS | this study | Used in FRET | |
| Genetic reagent (M. musculus) |
pcDNA 3.1(+) NLS-mRanBP1 |
this study | Used in BiFC | |
| Genetic reagent (M. musculus) |
pcDNA 3.1(+) NLS-NESmut |
this study | Used in BiFC | |
| Antibody | Rabbit anti-HA | Cell Signalling | 3724 | |
| Antibody | Mouse anti-Myc | ProteinTech | 60003–2-Ig | |
| Antibody | Alexa 647 labelled Anti-Rabbit IgG |
ThermoFisher | A-21244 | |
| Antibody | Dylight 405 labelled Anti-mouse IgG |
Beyontime | A0609 | |
| Antibody | Anti-GST | Santa Cruz | sc-138 | |
| Antibody | Anti-RanBP1 | ProteinTech | 27804–1-AP | |
| Antibody | Anti-CRM1 | absin | 115104 | |
| Antibody | Anti-NFκB | SAB | 48676–1 | |
| Chemical compound | Digitonin | Abcam | ab141501 |