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. 2019 May 7;8:e44352. doi: 10.7554/eLife.44352

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© 2019, Chen et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — The genes were coexpressed in E. coli Bl21-DE3-Star together with the P450 reductase gene RedB. Crude protein extracts were incubated with unlabeled or labeled (E,E)-FDP and volatile enzyme products were collected from the headspace of the assays using PDMS tubes. Product analysis was performed with GC-TDU-MS. See also Figure 5—figure supplement 1.

Figure 5—figure supplement 1. — The genes were coexpressed in E. coli Bl21-DE3-Star together with the P450 reductase gene RedB. Crude protein extracts were incubated with unlabeled or labeled (E,E)-FDP and volatile enzyme products were collected from the headspace of the assays using PDMS tubes. Product analysis was performed with GC-TDU-MS. See also Figure 5—figure supplement 1.