Figure 6. Developmental phenotype of the DdTPS8 knockout mutant.

Wild type AX4 cells (WT) and mutant DdTPS8 cells (mutant) were grown separately in HL5 to the log phase, washed in buffer, and plated clonally on dark nitrocellulose filters. The filters were cut in half and placed next to each other in one dish. The cells were incubated in the dark at 22°C and photographed from above with a dissecting microscope at the indicated times. This experiment was independently performed three times with same results and the data shown represent one of the replicates.