Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (C. elegans) | kin-19 | Wormbase | WBGene00002202 | |
| Gene (C. elegans) | rho-1 | Wormbase | WBGene00004357 | |
| Strain (E. coli) | OP50-1 | Caenorhabditis genetic center (CGC) |
RRID:WB-STRAIN:OP50-1 | Streptomycin resistant strain of OP50 |
| Genetic reagent (C. elegans) |
muEx473[Pkin-19::
kin-19::tagrfp + Ptph-1::gfp] |
David et al., 2010 | RRID:WB-STRAIN:CF3166 | N2 background |
| Genetic reagent (C. elegans) |
muEx512[Pkin-19::
tagRFP + Ptph-1::GFP] |
David et al., 2010 | CF3317 | N2 background |
| Genetic reagent (C. elegans) |
muIs209[Pmyo-3::kin-19::
tagrfp + Ptph-1::gfp] |
David et al., 2010 | CF3649 | N2 background |
| Genetic reagent (C. elegans) |
muIs210[Pmyo-3::
tagrfp + Ptph-1::GFP] |
David et al., 2010 | CF3650 | N2 background |
| Genetic reagent (C. elegans) |
muEx587[Pkin-19::
kin-19::meos2 + Punc-122::gfp] |
This paper | CF3706 | N2 background |
| Genetic reagent (C. elegans) |
uqIs9[Pmyo-2::rho-1::
tagrfp + Ptph-1::gfp] |
This paper | DCD13 | N2 background |
| Genetic reagent (C. elegans) |
uqEx4[Pmyo-3::kin-19::meos2] | This paper | DCD69 | N2 background |
| Genetic reagent (C. elegans) |
uqEx[Pmyo-2::rho-1::
meos2 + Punc-122:: gfp + cb-unc-119(+)] |
This paper | DCD83 | injected into EG6699 containing ttTi5605II; unc-119(ed3)III |
| Genetic reagent (C. elegans) |
uqEx22[Punc-54::rho-1::venus] | This paper | DCD127 | N2 background |
| Genetic reagent (C. elegans) |
uqIs12[Pmyo-2::rho-1::venus] | This paper | DCD146 | N2 background |
| Genetic reagent (C. elegans) |
uqEx37[Pkin-19::
kin-19::venus + Punc-122::gfp] |
Lechler et al., 2017 | DCD179 | N2 background |
| Genetic reagent (C. elegans) |
uqIs22 [Pkin19::kin19::
hisavi + Pkin19::birAtagrfp] |
This paper | DCD242 | fem-1(hc17ts) IV background |
| Genetic reagent (C. elegans) |
uqIs19 [Pmyo2::rho1::
hisavi + Pmyo2::birAtagrfp] |
This paper | DCD243 | fem-1(hc17ts) IV background |
| Genetic reagent (C. elegans) |
uqEx49[Pkin-19::meos] | This paper | DCD245 | N2 background |
| Genetic reagent (C. elegans) |
uqEx51[Pmyo-2::venus] | This paper | DCD248 | N2 background |
| Genetic reagent (C. elegans) |
rmIs126[Punc-54::YFP] | https://doi.org/10.1073/pnas.152161099 | RRID:WB-STRAIN:AM134 | N2 background |
| Genetic reagent (C. elegans) |
fem-1(hc17ts)IV | CGC | WB Cat# BA17, RRID:WB-STRAIN:BA17 |
|
| Recombinant DNA reagent |
tagRFP | Evrogen, pTagRFP-N, FP142 |
||
| Recombinant DNA reagent |
pKA1062 containing mEOS2 |
Other | Kaveh Ashrafi, UCSF, USA |
|
| Antibody | Phalloidin-iFluor 488 conjugate |
ABD-23115, AAT Bioquest, Biomol, Germany |
‘1:50’ | |
| Chemical compound, drug |
X34 | SML1954, Sigma- Aldrich, Germany |
1 mM final | |
| Chemical compound, drug |
thioflavin T | #ab120751, abcam, UK |
50 µM | |
| Commercial assay or kit |
5-Hydroxytryptamine creatinine sulfate complex |
H7752, Sigma-Aldrich, Germany |
10 µM | |
| Commercial assay or kit |
Nickel Sepharose 6 Fast Flow beads from HisTrap FF Crude column |
GE Healthcare, Uppsala, Sweden |