KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mCherry/RFP, guinea pig polyclonal | Fuchs lab | N/A |
| Anti-K6, rabbit polyclonal | Fuchs lab | N/A |
| Anti-K5, guinea pig polyclonal | Fuchs lab | N/A |
| Anti-Loricrin, Rabbit polyclonal | Fuchs lab | N/A |
| Anti-K10, rabbit polyclonal | Covance | PRB-159P |
| Anti-K14, chicken polyclonal | Covance | SIG-3476 |
| Biotin conjugated anti-CD45, rat monoclonal (Clone 30-F11) | Biolegend | Cat#103104 |
| Biotin conjugated anti-CD31, rat monoclonal (Clone MEC13.3) | Biolegend | Cat#102504 |
| Biotin conjugated anti-CD117, rat monoclonal (Clone 2B8) | Biolegend | Cat#105804 |
| Biotin conjugated anti-CD140a, rat monoclonal (Clone APA5) | Biolegend | Cat#135910 |
| Biotin conjugated anti-Thy1.1, mouse monoclonal (Clone OX7) | Biolegend | Cat#202510 |
| PE/Cy7 anti-mouse/human CD44, rat monoclonal (Clone IM7) | Biolegend | Cat#103030 |
| PerCP/Cy5.5 anti-mouse CD49f, rat monoclonal (Clone GoH3) | Biolegend | Cat# 313617 |
| Alexa Fluor® 700 anti-mouse CD45 rat monoclonal (Clone 30-F11) | Biolegend | Cat# 103128 |
| PE/Cy7 anti-mouse CD4 rat monoclonal (Clone GK1.5) | Biolegend | Cat# 100421 |
| APC/Cy7 anti-mouse CD8b rat monoclonal (Clone YTS156.7) | Biolegend | Cat# 126619 |
| Brilliant Violet 605™ anti-mouse CD19 rat monoclonal (Clone 6D5) | Biolegend | Cat# 115539 |
| Brilliant Violet 421™ anti-mouse CD25 rat monoclonal (Clone PC61) | Biolegend | Cat# 102033 |
| PE/Cy5 anti-mouse NK-1.1 mouse monoclonal (Clone PK136) | Biolegend | Cat# 108715 |
| PerCP/Cy5.5 anti-TCR β Hamster monoclonal (Clone H57–597) | Biolegend | Cat# 109227 |
| FITC anti-mouse CD80 Hamster monoclonal (Clone 16–10A1) | Biolegend | Cat# 104705 |
| FITC anti-mouse Foxp3 Rat monoclonal (Clone FJK-16s) | eBioscience | Cat# 11-5773-82 |
| Anti-Integrin-a6/CD49f, rat monoclonal (Clone GoH3) | eBioscience | Cat# 14-0495 |
| PE anti-TNF alpha rat monoclonal (Clone MP6-XT22) | eBioscience | Cat# 12-7321-41 |
| FITC Anti-Mouse CD34 rat monoclonal (Clone RAM34) | eBioscience | Cat# 11-0341-82 |
| APC Anti-Mouse OVA257–264 (SIINFEKL)/MHCI Mouse monoclonal (Clone eBio25-D1.16) | eBioscience | Cat# 17-5743 |
| PE anti- IFNγ rat monoclonal (Clone XMG1.2) | eBioscience | Cat# 12-7311-41 |
| PE anti- Granzyme B rat monoclonal (Clone NGZB) | eBioscience | Cat# 12-8898-80 |
| Anti-CD3e Hamster monoclonal (Clone 145–2C11) | eBioscience | Cat# 16-0031-82 |
| Anti- CD28 monoclonal (Clone 37.51) | eBioscience | Cat# 16-0281-82 |
| Anti-CD8 alpha rat monoclonal (Clone YTS 169.4) | BioXCell | Cat# BE0117 |
| Anti-CTLA-4 (CD.152) Hamster monoclonal (Clone UC10–4F10–11) | BioXCell | Cat# BE0032 |
| Anti-TGFβ monoclonal (Clone 1D11.16.8) | BioXCell | Cat# BE0057 |
| Anti-Ly6G/Ly6C(Gr1) monoclonal (Clone RB6–8C5) | BioXCell | Cat# BE0075 |
| Anti-CSF1 R(CD115) monoclonal (Clone AFS98) | BioXCell | Cat# BE0213 |
| Anti- Mouse B7–1/CD80 Goat polyclonal | R&D | Cat# AF740 |
| Anti- Human B7–1/CD80 Goat polyclonal | R&D | Cat# AF140 |
| Anti-Anti-CD80 Rabbit polyclonal | Abcam | Cat# ab64116 |
| Anti-CD8 rat monoclonal (Clone YTS169.4) | Abcam | Cat# ab22378 |
| Anti-Cleaved Caspase-3(Asp175) Rabbit monoclonal(Clone 5A1E) | Cell Signaling | Cat# 9664 |
| Anti-Phospho-Smad2 (Ser465/467) Rabbit monoclonal(Clone 138D4) | Cell Signaling | Cat# 3108 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma | Cat#T5648 |
| Diptheria toxin | Sigma | Cat# D0564 |
| Cyclophosphamide | Sigma | Cat# PHR1404 |
| 7,12-Dimethylbenz[a]anthracene | Sigma | Cat# D3254 |
| 12-O-T etradecanoylphorbol 13-acetate | Sigma | Cat# P1585 |
| Matrigel Matrix (High Concentration) | Corning | Cat# 354262 |
| OVA 257–264 | InvivoGen | Cat# vac-sin |
| Recombinant Mouse IL-2 | R&D | Cat# 402-ML-020 |
| Recombinant Human IL-2 | PeproTech | Cat# 200–02 |
| Critical Commercial Assays | ||
| CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry | Thermo | Cat# C34554 |
| Leukocyte Activation Cocktail with GolgiPlug TM | BD | Cat# 550583 |
| Nextera DNA Sample Preparation Kit | Illumina | Cat# FC-121-1030 |
| Alexa Fluor™ 647 Tyramide SuperBoost™ Kit | Thermo | Cat# B40926 |
| Agencourt AMPure XP | Beckman Coulter | Cat# A63880 |
| Foxp3 / Transcription Factor Staining Buffer Set | eBioscience | Cat# 00-5523-00 |
| MagCollect Streptavidin Ferrofluid | R&D | Cat# MAG999 |
| MojoSort™ Mouse CD8 T Cell Isolation Kit | Biolegend | Cat# 480035 |
| Deposited Data | ||
| Single cell RNaseq data | This Study | GEO: GSE108679 |
| Experimental Models: Cell Lines | ||
| Mouse SCC PDVC57 | (Bremner and Balmain, 1990) | N/A |
| Human SCC A431 | (Yang et al., 2015) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: TRE-OVA | (Rosenblum et al., 2011) | N/A |
| Mouse: TRE-HrasG12V | (Chin et al., 1999) | N/A |
| Mouse: C57BL/6J | The Jackson Laboratory | 000664 |
| Mouse: B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | The Jackson Laboratory | 007905 |
| Mouse: B6;129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J | The Jackson Laboratory | 024857 |
| Mouse: B6(Cg)-Rag2tm1.1Cgn/J | The Jackson Laboratory | 008449 |
| Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J | The Jackson Laboratory | 003831 |
| Mouse: B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) | The Jackson Laboratory | 002014 |
| Mouse: B6.129(Cg)-Foxp3tm3(DTR/GFP)Ayr/J | The Jackson Laboratory | 016958 |
| OligonucleOT-Ides | ||
| Mouse Cd80 sgRNA1: CATCAATACGACAATTTCCC | This Study | N/A |
| Mouse Cd80 sgRNA2: CGTGTCAGAGGACTTCACCT | This Study | N/A |
| Mouse Cd80 shRNA1: CCGAGTATAAGAACCGGACTT | This Study | N/A |
| Mouse Ctla4 shRNA1: GGAGCATGAACAGAGAGCT | This Study | N/A |
| Recombinant DNA | ||
| pLKO-rtTA-P2A-mCherry | This Study | N/A |
| pLKO-PGK-rtTA-SBE-mCherry | This Study | N/A |
| pLKO- PGK-rtTA-SBE-CreER | This Study | N/A |
| pLKO-PGK-rtTA-SBE-mCherry-P2A-CreER-U6-Scr Control sgRNA | This Study | N/A |
| pLKO-PGK-rtTA-SBE-mCherry-P2A-CreER-U6-Cd80 sgRNA | This Study | N/A |
| pMKO.1-Thy1.1-Scr Control shRNA | This Study | N/A |
| pMKO.1-Thy1.1-Ctla4 shRNA | This Study | N/A |
| Software and Algorithms | ||
| Fiju (ImageJ) | https://fiji.sc/ | N/A |
| Bowtie2 | (Langmead and Salzberg, 2012) | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| RSEM | (Li and Dewey, 2011) | http://deweylab.github.io/RSEM/ |
| DESeq | (Anders and Huber, 2010) | http://bioconductor.org/packages/release/bioc/html/DESeq.html |
| Gene Set Enrichment Analysis | (Subramani an et al., 2005) | http://software.broadinstitute.org/gsea/index.jsp |
| Mclust (R package) | (Fraley and Raftery, 2002) | https://cran.r-project.org/ |
| Picard | Broad Institute | https://github.com/broadinstitute/picard/releases/tag/2.7.1 |
| FlowJo | https://www.flowjo.com | N/A |
| Other | ||
| BD FACSAria II Cell Sorter | BD Biosciences | N/A |
| BD LSR-Fortessa analyzer | BD Biosciences | N/A |
| Illumina Nexseq 500 instrument | Illumina | N/A |