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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 Apr 12;1861(6):1180–1188. doi: 10.1016/j.bbamem.2019.04.006

Fig. 7.

Fig. 7.

Effect of 2-APB on Ca2+ responses evoked by carbachol or a 5 ns pulse. In (A-C), cells were pretreated with 100 μM 2-APB for 15 min prior to the experiment, which was carried out in the continued presence of 2-APB (gray bar). (A) Averaged fluorescence traces ± s.e. (n = 15) showing the lack of a Ca2+ response to carbachol (500 μM), which was applied by a pressure ejection pipette (arrow). (B) Averaged Ca2+ responses ± s.e. (n = 24) evoked by a 5 ns pulse (arrow). Error bars not shown are within the thickness of the line. (C) Fluorescence traces of the two cells that showed Ca2+ spikes evoked by the pulse (arrow).