| PCR cycling condition (30 cycles) | ||
| Initial denaturation | 98 °C | 1 min |
| Denaturation | 98 °C | 10 s |
| Annealing | 60 °C | 10 s |
| Extension | 72 °C | 5 min |
| Final extension | 72 °C | 10 min |
| 4 °C | ∞ | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.