| Antibodies |
| Anti-dihydropyridine-MDA-lysine adduct mouse mAb 1F83 |
Reference (Yamada et al., 2001) |
N/A |
| Anti-8-OH-dG antibody |
Abcam |
ab62623 |
| Anti-xCT antibody |
Abcam |
ab37185 |
| Anti-cyclooxygenase 2 (COX-2) antibody |
Abcam |
ab15191 |
| Goat anti-mouse IgG H&L (Alexa Fluor 647) |
Abcam |
ab150115 |
| Goat anti-rabbit IgG H&L highly cross-adsorbed secondary antibody (Alexa Fluor488) |
Thermo Fisher Scientific |
A11034 |
| Donkey anti-Goat IgG (H+L) cross-adsorbed, Alexa Fluor 647, polyclonal, secondary antibody |
Thermo Fisher Scientific |
A21447 |
| Anti-15-F2T-isoprostane purified IgG |
Oxford Biomedical Research |
IS20 |
| Anti-α-tubulin antibody (DM1A) |
Santa Cruz Biotechnology |
sc-32293 |
| Anti-xCT/SLC7A11 (D2M7A) antibody |
Cell Signaling Technology |
12691 |
| Anti-caspase-3 antibody |
Cell Signaling Technology |
9662 |
| Chemicals |
| (Poly(ethylene glycol) methyl ether-block-poly(lactide-co-glycolide)(PEG-PLGA) |
Sigma-Aldrich |
900948 |
| β-mercaptoethanol |
Sigma-Aldrich |
M3148 |
| Imidazole ketone erastin (IKE) |
Reference(Larraufie et al., 2015) |
N/A |
| Ferrostatin-1 (fer-1) |
Reference(Skouta et al., 2014) |
N/A |
| Critical Commercial Assays |
| GSH/GSSG Ratio Detection Assay Kit |
Abcam |
ab13881 |
| TBARS Assay Kit |
Cayman |
700870 |
| BODIPY™ 581/591 C11 |
Thermo Fisher Scientific |
D3861 |
| RiboPure™ RNA Purification Kit |
Thermo Fisher Scientific |
AM1924 |
| CellTiter-Glo Luminescent Cell Viability Assay |
Promega |
G7573 |
| RNAeasy extraction kit |
QIAGEN |
74106 |
| QIAshredder |
QIAGEN |
79656 |
| High Capacity cDNA Reverse Transcription Kit |
Thermo Fisher Scientific |
4368814 |
| Power SYBR Green PCR Master Mix |
Thermo Fisher Scientific |
4368702 |
| Cell culture reagents and others |
| ProLong™ Gold anti-fade Mount with DAPI |
Thermo Fisher Scientific |
P36962 |
| 10% goat serum |
Thermo Fisher Scientific |
50197Z |
| Donkey serum |
Sigma-Alderich |
D9663 |
| Poly-lysine |
Sigma-Aldrich |
P4832 |
| Fetal bovine serum |
Thermo Fisher Scientific |
10437–028 |
| DMEM |
Corning |
10–013 |
| Fetal bovine serum |
Life Technologies |
10437036 |
| RPMI-1640 |
ATCC |
30–2001 |
| Non-essential amino acids |
Thermo Fisher Scientific |
11140076 |
| Penicillin-streptomycin mix |
Thermo Fisher Scientific |
15140148 |
| IMDM |
Thermo Fisher Scientific |
12440053 |
| HBSS |
Thermo Fisher Scientific |
14025076 |
| Steriflip filter unit |
Thomas Scientific |
1189Q46 |
| Amicon Ultra-15 centrifugal filter units |
Millipore |
UF9050 |
| K3 EDTA micro tube |
SARSTEDT |
41.1504.105 |
| RIPA buffer |
Thomas Scientific |
89900 |
| Software and Algorithms |
| ChemDraw Ultra, Version 14.0 |
Perkin Elmer |
http://www.perkinelmer.com/category/chemdraw |
| Prism, Version 7.0 |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/ |
| LipoStar, Version 1.0.4 |
Molecular Discovery |
https://www.moldiscovery.com/software/lipostar/ |
| MetaboAnalyst, Version 4.0 |
MetaboAnalyst |
https://www.metaboanalyst.ca/faces/docs/About.xhtml |
| MassLynx, Version 4.1 |
Waters |
http://www.waters.com/waters/en_US/MassLynx-Mass-Spectrometry-Software-/nav.htm?cid=513164&locale=en_US |
| XCMS package, Version 3.2.0 |
Bioconductor |
http://packages.renjin.org/package/org.renjin.bioconductor/xcms |
