Extended Data Table 1. Collection, refinement and validation of cryo-electron-microscopy data.
70S d2d8 stapled (EMDB-0261) (PDB-6HRM) |
|
---|---|
Data collection and processing | |
Magnification | 134615x |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 26.85 |
Defocus range (μm) | -2.0 to -3.5 |
Pixel size (Å) | 1.06 |
Symmetry imposed | none |
Initial particle images (no.) | 306214 |
Final particle images (no.) | 94371 |
Map resolution (Å) FSC threshold |
3.0 0.143 |
Map resolution range (Å) | 2.2-8.8 |
Refinement | |
Initial model used (PDB code) | 5MDZ |
Model resolution (Å) FSC threshold |
3.1 0.143 |
Model resolution range (Å) | 2.6-7.1 |
Map sharpening B factor (Å2) | -78.8 |
Model composition Non-hydrogen atoms Protein residues Nucleic acid residues Ligands |
145181 6131 4563 453 |
B factors (Å2) Protein RNA |
86.4 55.1 |
R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.008 1.004 |
Validation MolProbity score Clashscore Poor rotamers (%) |
1.45 2.96 0.47 |
Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
94.78 5.16 0.05 |