. Author manuscript; available in PMC: 2019 Jun 1.

Published in final edited form as: Nature. 2018 Dec 5;564(7736):444–448. doi: 10.1038/s41586-018-0773-z

Extended Data Table 1. Collection, refinement and validation of cryo-electron-microscopy data.

	70S d2d8 stapled (EMDB-0261) (PDB-6HRM)
Data collection and processing
Magnification	134615x
Voltage (kV)	300
Electron exposure (e–/Å²)	26.85
Defocus range (μm)	-2.0 to -3.5
Pixel size (Å)	1.06
Symmetry imposed	none
Initial particle images (no.)	306214
Final particle images (no.)	94371
Map resolution (Å) FSC threshold	3.0 0.143
Map resolution range (Å)	2.2-8.8

Refinement
Initial model used (PDB code)	5MDZ
Model resolution (Å) FSC threshold	3.1 0.143
Model resolution range (Å)	2.6-7.1
Map sharpening B factor (Å²)	-78.8
Model composition Non-hydrogen atoms Protein residues Nucleic acid residues Ligands	145181 6131 4563 453
B factors (Å²) Protein RNA	86.4 55.1
R.m.s. deviations Bond lengths (Å) Bond angles (°)	0.008 1.004
Validation MolProbity score Clashscore Poor rotamers (%)	1.45 2.96 0.47
Ramachandran plot Favored (%) Allowed (%) Disallowed (%)	94.78 5.16 0.05