Table 3.
Multilineage Hematopoietic Engraftment in Primary Recipient Mice
| Experiment No. (CS) | Tissue | Blood (%) |
Bone Marrow (%) |
Spleen (%) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Myeloid | B | T | NK | Myeloid | B | T | NK | Myeloid | B | T | NK | ||
| B (16) | Anterior | 28 | 30 | 40 | 0 | 8 | 90 | 0.3 | 0.2 | 3.9 | 88.2 | 0.7 | 0.1 |
| C (17) | Posterior | 29 | 69 | 0 | 0 | 42 | 47 | 1.5 | 0 | 32 | 62 | 5 | 0.5 |
| 25 | 70 | 0 | 0 | 43 | 47 | 3.9 | 0.6 | 9 | 78 | 1.3 | 1.4 | ||
| 7 | 45 | 45 | 2 | 34 | 52 | 8 | 4 | 30 | 8 | 60 | 1 | ||
| D (17) | Whole | NA | NA | NA | NA | 56 | 33 | 1 | 0 | 22 | 43 | 0 | 0 |
| F (16) | Part 2 | 8 | 61 | 30 | 0 | 23 | 75 | 1.5 | 0.1 | 26 | 69 | 0.9 | 3 |
| Part 3 | 20 | 46 | NA | 0 | 30 | 69 | 0.7 | 0 | 20 | NA | NA | NA | |
| G (16) | Part 2 | 51 | 39 | 2 | 0 | 68 | 26 | 0.1 | 0.3 | 33 | 55 | 7 | 3 |
| Part 3 | 33 | 17 | 33 | 12 | 82 | 12 | 0.3 | 0.9 | 33 | 49 | 7 | 7 | |
| I (17) | Part 2 | 10 | 72 | 11 | 2.3 | 23 | 68 | 1.0 | 0.7 | 6 | 79 | 10 | 3.6 |
| Part 3 | 36 | 47 | 1.4 | 0.8 | 31 | 61 | 1 | 0.8 | 10 | 84 | 1.4 | 0.8 | |
| Part 4 | 16 | 61 | 2.0 | 7 | 18 | 70 | 1.3 | 0.1 | 9 | 85 | 0.7 | 0 | |
| Liver | 12 | 22 | 48 | 17 | 70 | 18 | 12 | 2.6 | 18 | 38 | 43 | 10 | |
Percentage of myeloid cells (CD33+), B cells (CD19+), T cells (CD3+), and NK cells (CD94+) in the human CD45+ cell population in the peripheral blood, bone marrow, and spleen of repopulated primary recipient NSG mice is shown. Only repopulated mice are shown and correspond to engrafted mice indicated in Tables 1 and 2. The NSG mouse engrafted with embryonic liver is included for completeness. NA, not assessed.