Fig. 1.

Replisome responses to leading-strand polymerase stalling at different types of DNA damage. (a) Schematic of templates with leading-strand DNA damage and the architecture of replication products formed in the absence of leading-strand re-priming. In this and all subsequent figures, red represents leading strands, and blue represents lagging strands; the position of the ARS306 origin of replication is marked, Ori. (b) Replication products from undamaged and damaged (THF, AB, or CPD) templates. (c–d) Two-dimensional gel analysis of the reaction products from undamaged (c) and THF (d) templates at a 60-min time point in (b). See also Supplemental Fig. S1.