Fig. 3.
Replisomes slow down after leading-strand polymerase stalling. (a) Schematic of CPDLEAD templates used in this figure. (b and c) Pulse-chase experiments on 3-kb CPDLEAD (b) and 4.5-kb CPDLEAD templates (c). The chase was added at 2 min 50 s. Pol δ was omitted because elevated nucleotide concentrations during the chase promote excessive strand-displacement synthesis. (d) Schematic of undamaged template used in panel e. (e) Pulse-chase experiment performed as in panels b and c. (f) Quantification of experiments performed as in panel e. Error bars represent the SEM from three experiments. See also Supplemental Fig. S3.