Table 1.
Oligonucleotide primers used in experimental design
| Primer name | Primer Sequence | Use |
|---|---|---|
| Ov2 ovhv2-miR-17-10 binding site mutagenesis Forward | GCTCCTGTAACCTCTTCTTAAAATTGCGTGATGCCCTTCTATTTGTGCTCCTGC | mutation of Ov2 ovhv2-miR-17-10 binding site |
| Ov2 ovhv2-miR-17-10 binding site mutagenesis Reverse | GCAGGAGCACAAATAGAAGGGCATCACGCAATTTTAAGAAGAGGTTACAGGAGC | mutation of Ov2 ovhv2-miR-17-10 binding site |
| Ov2 ovhv2-miR-61-1 binding site mutagenesis Forward | CATCCACTCCCACCTACCTGAGGTGACCATAAATGTTGTTCGGAGTTGTAAAAATCATTTGT | mutation of Ov2 ovhv2-miR-61-1 binding site |
| Ov2 ovhv2-miR-61-1 binding site mutagenesis Reverse | ACAAATGATTTTTACAACTCCGAACAACATTTATGGTCACCTCAGGTAGGTGGGAGTGGATG | mutation of Ov2 ovhv2-miR-61-1 binding site |
| Ov2 Fwd | GGATCCACCATGTCCGATAATAAAAAGC | For cloning into pcDNA3.1+ |
| Ov2 Rev | GAATTCACGCGTCTACAAGCTGTGTAACATATTCAACTCC | For cloning into pcDNA3.1+ |
| Ov2 BglII fwd | TCAGATCTCATGTCCGATAATAAAAAGC | For cloning into pEGFPN1 |
| Ov2 Ecor1 Ov2 rev | GAATTCGCAAGCTGTGTAACATATTCAACTCC | For cloning into pEGFPN1 |