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. 2019 May 17;9:7539. doi: 10.1038/s41598-019-44048-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IQGAP1 interacts with DVL2 and translocates DVL2 into the nucleus. (A) Co-IP study of non-transfected HEK293T, SW1990 and CFPAC-1 cells confirmed that IQGAP1 and DVL2 can bind with each other. (B) Co-IP revealed that both IQGAP1 and DVL2 bind to β-catenin. (C) Protein expression of DVL2 was decreased by the suppression of IQGAP1, and overexpression of IQGAP1 had the opposite effect. (D) mRNA levels of DVL2 determined by qRT-PCR were not changed by the suppression or overexpression of IQGAP1. (E) Immunofluorescence further revealed the change in protein expression and distribution of DVL2 by IQGAP1 knockdown. (F) IQGAP1 translocated DVL2 into the nucleus. DVL2 expression in PANC-1 cells transfected with IQGAP1 expression plasmid. Arrows indicate representative cells. Nuclei were visualized by DAPI staining. Uncropped western blots are shown in Supplementary Fig. 2.