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. 2019 May 17;9:7539. doi: 10.1038/s41598-019-44048-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IQGAP1 activates the Wnt/β-catenin signaling pathway and promotes β-catenin translocation into the nucleus. Total β-catenin, cyclin D1and c-myc protein (A) and mRNA levels (B) were assayed by western blotting and qRT-PCR, respectively, in IQGAP1 knockdown, IQGAP1 overexpression, and control groups. (C,D) Protein expression of cytoplasmic or nuclear β-catenin and its target genes (cyclin D1 and c-myc) in IQGAP1 knockdown, IQGAP1 overexpression and control groups was assayed by western blotting. (E) Immunofluorescence further revealed the change in protein expression and distribution of β-catenin by IQGAP1 knockdown. Nuclei were visualized by DAPI staining. (F) IQGAP1 translocates β-catenin into the nucleus. β-catenin expression in PANC-1 cells transfected with IQGAP1 expression plasmid. Arrows indicate representative cells. (G) The interaction between β-catenin and DVL2 was determined in SW1990 and CFPAC-1 knockdown cells by Co-IP assay. *P < 0.05, **P < 0.01, and ***P < 0.001. Uncropped western blots are shown in Supplementary Fig. 2.