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. 2019 May 17;9:7533. doi: 10.1038/s41598-019-43862-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expressional change of hepatic srebp-1 genes (a) and their targeted-genes (b) between elovl2 knockout (KO) and wildtype (WT) salmon fed either low PUFA or standard commercial diet. (a) Boxplots displaying all four srebp-1 genes with their respective expression (log2 CPM) in KO and WT salmon fed either low PUFA or standard diet. Asterisks indicates differential expression (q < 0.05). (b) Violin plot showing 183 lipid genes with least 1 Srebp-1-targeted motif in the promoter region. The density of the plot is proportional to the number of genes in each log2 fold change unit. Both fish fed low PUFA or standard diet showed significant enrichment of the Srebp-1-targeted genes. Gene expression analysis was performed using n = 18 elovl2 KO + n = 18 WT fed low PUFA diet, and n = 11 each of elovl2 KO and WT fed standard diet.