Fig. 6.

Maternal Zelda overexpression causes partial embryonic lethal. a Quantitative RT-PCR experiments were used to analyze the effectiveness of zelda knockdown in 0.75-h stage embryos. The two-tailed Student’s t test was used to analyze statistical variance. Data expressed as means of 2 independent experiments. Error bars indicate mean ± s.d. **P < 0.01. b Relative hatching rate of embryos with indicated genotypes. The two-tailed Student’s t test was used to analyze statistical variance. Data expressed as means of 3 independent experiments. Error bars indicate mean ± s.d. ***P < 0.001. c Females with indicated genotype (uas-zelda RNAi/CyO; jumu¹/TM3) were crossed with males (nos-gvp/CyO;jumu²/TM3), the eggs produced by female progenies were then fertilized by the wild-type sperm. Unhatched embryos and larvae with indicated genotypes were counted separately. Source data are provided as a Source Data file. n.s. not significant