Table 2.

Statistical analysis corresponding to the factorial design performed

Dependent variable: survival rate (%)	R-squared: 0.977
Method: least squares	Adj. R-squared: 0.972
Number of observations: 12	F-statistic: 209.9
Degrees of freedom residuals: 10	Probability (F-statistic): 6.82 × 10−9
Degrees of freedom: 2
Coefficient for Temperature: − 0.534	Probability: 0.338
Coefficient for pH: 14.305	Probability: 0.001

A Python script was written for the analysis. Survival rate was the dependent variable, and pH and temperature the independent factors. The surface was fitted to a plane with intercept 0