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. 2019 May 17;9:7532. doi: 10.1038/s41598-019-43992-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dab2 promoter methylation in pancreatic tumor samples and pancreatic cancer cell lines. (a,c) Genomic DNA was isolated from the indicated pancreatic cancer cell lines and the normal or tumor pancreatic tissue samples as described under Methods. Following bisulfite modification of the DNA, methylation-specific PCR was performed using primers corresponding to unmethylated (U) and methylated (M) sequences derived from exon 2 of the Dab2 gene promoter. PCR products were separated on a 2% agarose gel and visualized by EtBr staining. (b) MiaPaCa2 cells were untreated or treated with 5-aza-2′deoxycytidine (Aza), trichostatin A (TSA) or the combination (Aza + TSA) as described in Methods. Following treatment, mRNA was isolated and qRT-PCR was performed to determine mRNA expression of Dab2 and E-cadherin. Relative expression is shown, with mRNA levels in untreated MiaPaCa2 cells set to 1.0. Shown is the mean relative expression level ± SD performed in triplicate. * signifies p < 0.05.