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. 2019 May 17;10(6):389. doi: 10.1038/s41419-019-1620-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a A streptavidin-capture assay was performed for A375 cells transfected with biotin-miR-NC or biotin-miR-34a, followed by a quantitative real-time polymerase chain reaction (qRT-PCR) assay to analyze MALAT1 and GAPDH mRNA levels. b An Ago2 immunoprecipitation experiment was completed for A375 cells transfected with control miRNA (miR-NC) or miR-34a, followed by a qRT-PCR assay to analyze the MALAT1 associated with Ago2. c Schematic representation of the wild-type miR-34a and mutated miR-34a. d Streptavidin -capture assay was performed for A375 cells transfected with biotin-miR-NC, biotin-miR-34a, or mutated biotin-miR-34a, followed by a qRT-PCR assay to analyze MALAT1 and GAPDH mRNA levels. e An Ago2 immunoprecipitation experiment was performed for A375 cells transfected with control miRNA (miR-NC), miR-34a, or mutated miR-34a, followed by a qRT-PCR assay to analyze the MALAT1 associated with Ago2