Fig. 5. In vivo confirmation that MALAT1 functions as a miR-34a sponge.

a Schematic diagram of the animal experimental design. The A375 cells stably expressing either sh-lncRNA-MALAT1 or sh-NC were injected subcutaneously into the dorsal flanks of the indicated numbers of BALB/c nude mice. (b–c) At 21 days after injection, the xenografts were dissected and examined in a quantitative real-time polymerase chain reaction (qRT-PCR) assay to analyze MALAT1 (b) and miR-34a (c) expression levels. d c-Myc and Met protein levels were analyzed in a western blot. e Schematic diagram of the animal experimental design. Stable A375 cells with pLVX-IRES-Puro-MALAT1 or pLVX-IRES-Puro-MALAT1-mut were injected subcutaneously into the dorsal flanks of 5-week-old male BALB/c nude mice. f Xenografts were dissected 21 days after injections, and MALAT1 and MALAT1-mut expression levels were analyzed in a qRT-PCR assay. g c-Myc and Met protein levels were analyzed in a western blot