Table 1.

Association of 96 genes encoding heat shock proteins with overall survival of breast cancer patients from TCGA and KM plotter.

HSP family	Gene	OS (low vs high expression)		HSP family	Gene	OS (low vs high expression)
		TCGA BRCA p-value	Kaplan_Meier plotter p-value			TCGA BRCA p-value	Kaplan_Meier plotter p-value
HSP70	HSPA1A	0,7898	0,0012**	HSP40	DNAJA1	0,1737	0,0104*
	HSPA1B	0,8009	0,0012**		DNAJA2	0,5296	0,062
	HSPA1L	0,2863	0,1784		DNAJA3	0,6468	0,7976
	HSPA2	0,00642**	0,00045***		DNAJA4	0,8641	0,19
	HSPA4	0,2382	0,1713		DNAJB1	0,1037	0,2173
	HSPA4L	0,9558	0,5283		DNAJB2	0,4024	0,0965
	HSPA5	0,642	0,3809		DNAJB3	N/A	N/A
	HSPA6	0,3907	0,478		DNAJB4	0,3863	0,6261
	HSPA7	0,6274	N/A		DNAJB5	0,03838*	0,6713
	HSPA8	0,04814*	0,0787		DNAJB6	0,7771	0,4362
	HSPA9	0,004824**	0,0562		DNAJB7	0,1804	0,0943
	HSPA12A	0,4018	0,7155		DNAJB8	N/A	0,2438
	HSPA12B	0,398	0,4443		DNAJB9	0,1394	0,2638
	HSPA13	0,8888	0,5764		DNAJB11	0,4576	0,958
	HSPA14	0,1735	0,1348		DNAJB12	0,6549	0,5622
HSP110	HSPH1	0,5553	0,054		DNAJB13	0,3657	0,0964
	HYOU1	0,445	0,5638		DNAJB14	0,4799	0,9681
HSPB	HSPB1	0,5245	0,003**		DNAJC1	0,7878	0,6448
	HSPB2	0,2765	0,5531		DNAJC2	0,5074	0,009**
	HSPB3	N/A	0,7777		DNAJC3	0,967	0,2065
	HSPB4	N/A	0,2363		DNAJC4	0,1096	0,0555
	HSPB5	0,05732	0,0211*		DNAJC5	0,1368	0,0294*
	HSPB6	0,5086	0,299		DNAJC5B	0,4402	0,8677
	HSPB7	0,2002	0,1558		DNAJC5G	N/A	0,0282*
	HSPB8	0,3012	0,7576		DNAJC6	0,07342	0,6954
	HSPB9	0,9359	0,1577		DNAJC7	0,719	0,304
	HSPB10	N/A	0,7791		DNAJC8	0,9024	0,6157
	HSPB11	0,4479	0,19		DNAJC9	0,6161	0,00000065****
HSPC (HSP90)	HSP90AA1	0,0009347***	0,0094**		DNAJC10	0,8865	0,4738
	HSP90AA3P	N/A	N/A		DNAJC11	0,4812	0,7795
	HSP90AB1	0,04601*	0,5055		DNAJC12	0,2195	0,0906
	HSP90B1	0,5217	0,3144		DNAJC13	0,01078*	0,5831
	HSP90L	0,1862	0,1497		DNAJC14	0,2178	0,2195
Chaperonins	BBS10	0,8031	0,2716		DNAJC15	0,5046	0,3538
	BBS12	0,4741	0,051		DNAJC16	0,2662	0,00000096****
	CCT1	0,009986**	0,000016****		DNAJC17	0,7579	0,0967
	CCT2	0,04282*	0,0000014****		DNAJC18	0,4269	0,6657
	CCT3	0,3363	0,0064**		DNAJC19	0,1643	0,0082
	CCT4	0,02085*	0,065		DNAJC20	0,04307*	0,0053**
	CCT5	0,09297	0,0064**		DNAJC21	0,445	0,6636
	CCT6A	0,002706**	0,00067***		DNAJC22	0,8126	0,7541
	CCT6B	0,6901	0,34		DNAJC23	0,001951**	0,056
	CCT7	0,1599	0,0119*		DNAJC24	0,7272	0,3404
	CCT8	0,223	0,00037***		DNAJC25	0,6785	0,8495
	HSPD1	0,5599	0,000004****		DNAJC26	0,2249	0,7234
	HSPE1	0,3572	0,0779		DNAJC27	0,9823	0,0038**
	MKKS	0,4983	0,4378		DNAJC28	0,3508	0,2808
					DNAJC29	0,9692	0,7073
					DNAJC30	0,2152	0,7974

Stratification into high-expression and low-expression groups was performed according to median expression of each HSP. P-value p ≤ 0,05 was considered as statistically significant. Six genes encoding HSPs marked in bold were identified as significantly associated with overall survival in both datasets and selected for further validation. *p ≤ 0,05; **p ≤ 0,01; ***p ≤ 0,001; ****p ≤ 0,0001.