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. 2019 May 17;9:7556. doi: 10.1038/s41598-019-43622-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generalised down-modulation of CD126 in leukocytes from patients with moderate-severe asthma. Monocytes (a), neutrophils (b), and lymphocytes (c,d) were gated on a flow cytometry FSC/SSC plot. Afterwards, lymphocytes were further subdivided into CD4⁻ (c) and CD4⁺ (d) cells. The number of CD126 antibodies bound per cell (ABC; median ± IQR1-3; see Material and Methods section) was measured on every population (a–d) in intermittent-mild (IMA; N = 102) and moderate-severe (MSA; N = 90) asthma patients. Statistically significant differences between these two groups of donors are indicated (t-test: P < 0.05).