Fig. 2.

Separation of nicotine, anatabine, and anabasine enantiomers from racemic alkaloid standards on the CHIRALPAK AGP column (A). R-nicotine and S-anatabine were distinguished and quantified by the differences in quantitation and confirmation transitions (m/z) for each (see Table 1). Separation of nornicotine enantiomers from the racemic nornicotine standard on the LUX Cellulose-2 column (B).