Table 1.
Mass spectrometric parameters for the quantification and confirmation of alkaloids in the multiple reaction monitoring (MRM) mode.
| Compound | Quantitation Transition (m/z) | Collision (V) | Confirmation Transition (m/z) | Collision (V) |
|---|---|---|---|---|
| (R)-Nicotine | 163 > 132 | 16 | 163 > 117 | 26 |
| (S)-Nicotine | 163 > 80 | 14 | 163 > 117 | 26 |
| (R,S)-Nicotine-d4 | 167 > 136 | 14 | 167 > 121 | 24 |
| (R,S)-Nornicotine | 149 > 117 | 20 | 149 > 80 | 18 |
| (R,S)-Nornicotine-d4 | 153 > 121 | 22 | 153 > 96 | 24 |
| (R,S)-Anabasine | 163 > 92 | 20 | 163 > 80 | 18 |
| (R,S)-Anatabine | 161 > 107 | 12 | 161 > 80 | 28 |