Table 2.

Parameters, experimental runs and responses of Placket-Burman design used for the selection of significant parameters for laccase production in solid state fermentation using Bacillus sp. MSK-01.

Run	Time (h)	Temp (°C)	pH	CuSO4 (μM)	Moisture ratio	MgSO4 (μM)	MnSO4 (μM)	yeast extract (%)	Moistening solution	Tween -80 (%)	Inoculum (%)	Activity (IU/g)
1	96	37	9	0	1:1	0	100	0	1	0.1	10	105.75
2	48	37	9	100	1:1	0	0	0	0	0.1	20	39.66
3	96	30	7	0	1:2	0	100	0	0	0.1	20	73.67
4	96	30	9	100	1:1	100	100	0	0	0.0	10	84.33
5	48	37	7	100	1:2	0	100	0	1	0.0	10	26.67
6	48	37	9	0	1:2	100	100	0	0	0.0	20	25.58
7	96	37	7	0	1:1	1000	0	0	1	0.0	20	91.42
8	96	30	9	100	1:2	0	0	0	1	0.0	20	76.16
9	48	30	7	0	1:1	0	0	0	0	0.0	10	0.17
10	48	30	7	100	1:1	100	100	0	1	0.1	20	0.12
11	96	37	7	100	1:2	100	0	0	0	0.1	10	160.91
12	48	30	9	0	1:2	100	0	0	1	0.1	10	93.34